Keratocyte Apoptosis and Failure of Corneal Allografts

Keratocyte apoptosis and failure of corneal allografts.

BACKGROUND Murine models of high-risk and low-risk corneal transplantation were used to determine the role of keratocyte apoptosis in the failure of orthotopic allogeneic corneal transplants. MATERIALS AND METHODS Normal (low-risk, low-rejecting) and inflamed/vascularized (high-risk, high-rejecting) BALB/c recipient beds received fully mismatched C57BL/6 corneal allografts. Apoptosis was dete...

Keratocyte apoptosis after corneal surgery.

PURPOSE Programmed cell death (apoptosis) is the controlled death of cells that occurs with minimal collateral damage to surrounding cells or tissue during development, homeostasis, and wound healing. The authors hypothesize the keratocyte apoptosis is an initiating factor in the wound-healing response after refractive surgical procedures. To evaluate the effects of different corneal manipulati...

Keratocyte apoptosis increases in human corneal allografts during immune-mediated graft rejection.

Twist2: role in corneal stromal keratocyte proliferation and corneal thickness.

PURPOSE Twist2 is a member of a family of bHLH transcription factors critical for normal mesenchymal proliferation and differentiation. In this study, the authors analyzed the role of Twist2 in the eye and cornea through examination of a Twist2 loss-of-function mouse mutant. METHODS Twist2 expression during eye development in the mouse was investigated using RT-PCR and mRNA slide in situ hybr...

Delay of corneal epithelial wound healing and induction of keratocyte apoptosis by platelet-activating factor.

PURPOSE To examine the role of the lipid mediator platelet-activating factor (PAF) in epithelial wound healing. METHODS A 7-mm central de-epithelializing wound was produced in rabbit corneas, and the tissue was incubated with 125 nM carbamyl PAF (cPAF), an analogue of PAF. Rabbit corneal epithelial and stromal cells were also cultured in the presence of cPAF. Cell adhesion, proliferation, and...